Journal: Pharmaceutics

Article Title: A Novel 2-Methoxyestradiol Derivative: Disrupting Mitosis Inhibiting Cell Motility and Inducing Apoptosis in HeLa Cells In Vitro

doi: 10.3390/pharmaceutics16050622

Figure Lengend Snippet: Compound 4a markedly diminished the invasiveness of cervical cancer cells (HeLa). The anti-invasive potential of the test compound is illustrated by representative images ( B ), and is quantified by the percentage of invading cells in the Boyden chamber containing different concentrations of 4a , using an EMEM medium supplemented with 10% FBS as a chemoattractant ( A ). Results are presented as mean values ± SEM of the data from three separate measurements with duplicates. ** and *** indicate p < 0.01 and p < 0.001, respectively, compared to untreated control samples.

Article Snippet: The polyethylene terephthalate (PET) membrane (8 μm pore size) and the thin layer of the matrigel basement matrix in special Boyden chamber inserts (BioCoatTM Matrigel ® Invasion Chambers, Corning Inc., Corning, NY, USA) were prehydrated (2 h, serum-free EMEM) and placed onto a 24-well plate.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: LncRNA BCYRN1 as a Potential Therapeutic Target and Diagnostic Marker in Serum Exosomes in Bladder Cancer

doi: 10.3390/ijms25115955

Figure Lengend Snippet: Knockdown of BCYRN1 decreased the viability of BC cells. ( A ) Cell proliferation in two-dimensional monolayer culture was evaluated using XTT assays in BC cell lines. ( B ) Cell proliferation in 3D cell culture was evaluated using 3D spheroid assays in BC cell lines. The proliferation of BC cells was significantly reduced by the knockdown of BCYRN1 . ( C ) Cell migration ability was measured using wound healing assays. Migration ability was significantly reduced by the knockdown of BCYRN1 . ( D ) Cell invasion ability was measured using Matrigel invasion assays. The counted infiltrated cells were decreased in BCYRN1 -knockdown cells compared with those in mock-transfected cells. Each experiment was repeated at least three times. The error bars indicate standard errors of the means. * p < 0.0001. The relationships among the four groups were analyzed using multiple comparison tests with the Bonferroni–Dunn method.

Article Snippet: Matrigel invasion chambers (Corning Biocort, Bedford, MA, USA) with bottoms made from PET membranes (8.0 μm pores) and coated with a thin layer of Matrigel basement membrane matrix were used as cell culture inserts in 24-well tissue culture companion plates.

Techniques: Knockdown, Cell Culture, Migration, Transfection, Comparison

Journal: Cancer Medicine

Article Title: LRP5 knockdown: effect on prostate cancer invasion growth and skeletal metastasis in vitro and in vivo

doi: 10.1002/cam4.111

Figure Lengend Snippet: Effect of DN-LRP5 on PC-3 cells invasion, colony formation, and migration in vitro . Control (PC-3-pcDNA3.1) and experimental (PC-3-DN-LRP5) cells invasive capacity was evaluated by using a Boyden chamber Matrigel invasion assay. After 18 h of incubation, the invaded cells were fixed, stained, and 10 random fields were counted. Number of cells invading is shown as bar diagram ± SEM (A) as described in “Material and Methods”. Using soft agar assay, the ability of control and experimental cells to form colonies was examined and the number of colonies formed in each group was counted (B) as described in “Material and Methods”. PC-3 cells migration was determined by wound healing assay by seeding PC-3-pcDNA3.1 and PC-3-DN-LRPP5 cells in six-well plates and allowing them to grow as a monolayer and making a wound as described in “Material and Methods”. Control and experimental migrating cells were photographed at different time points (C). Percent wound healing was recorded at different time points, and percentage of wound healing with respect to T 0 was calculated using the equation described in “Material and Methods”. Results are presented as the mean ± SEM of two different experiments in duplicate from control and experimental cells. Significant differences from the control is represented by an asterisk ( P < 0.05).

Article Snippet: The invasive capacity of 5 × 10 4 PC-3-pcDNA3.1 and PC-3-DN-LRP5 cells were examined using two-compartment Boyden chamber Matrigel invasion assay (Costar Transwell, Corning Corporation, Corning, NY) as described previously .

Techniques: Migration, In Vitro, Invasion Assay, Incubation, Staining, Soft Agar Assay, Wound Healing Assay

Journal: Oncotarget

Article Title: Activation of TRPM7 by naltriben enhances migration and invasion of glioblastoma cells

doi: 10.18632/oncotarget.14496

Figure Lengend Snippet: ( A ) Representative images of wound healing following induction of scratch. Cells were treated with vehicle (0.1% DMSO; control; n = 6) or with naltriben (50 μM; n = 10), then images were captured at 0, 4, 8, and 12 hours, and gap closure was analyzed. ( B ) The wound closure of naltriben-treated cells at 4, 8, and 12 hours were significantly higher compared to the control group at the corresponding time points. ** represents p < 0.01 (Student's t-test ). ( C ) Representative images from Corning Biocoat Matrigel invasion assays to detect cell invasion in vitro . Cells were treated with vehicle (0.1% DMSO; control; n = 6) or with naltriben (50 μM; n = 6) for 12 hours. ( D ) Invasion of naltriben-treated cells at 12 hours was significantly higher compared to the control group. ** represents p < 0.01 (Student's t-test ).

Article Snippet: U87 cell invasion was evaluated according to the manufacturer's instructions for the Corning Matrigel invasion chambers (8-μm polycarbonate Nucleopore filters, cat# 354480, BD Biosciences).

Techniques: In Vitro